Figure 2.

HIV neutrophil H3K4me3-marked histones are characterized by large fluctuations within DNA annotation. (A) Pie chart analysis of separate samples within H3K4me3 in HIV and HCs showed only minor differences in DNA annotation of genomic regions. (B) Binding sites overlap analysis revealed 1,862 DNA sequences associated with H3K4me3 that specific characterize HIV patients. (C) Compression of HIV vs. HCs demonstrated the most important changes in exon, intron and promoter TSS genomic features. (D) Analysis of statistic differences in protein binding to the H3K4me3 region revealed that most of the binding sites in HIV neutrophils are upregulated. Left and bottom panel show statically significant differences in binding sites as enlarged region. Low panel displays ten peaks with highest statistically significant differences and lowest FDR. More details and all peaks with statistically significant differences are presented in the Supplementary Table S2 .