Table 1.

Primer information of IGF1 and a reference gene in geese.

Primer	Amplified	Primer sequence (5${}^{\prime }$–3${}^{\prime }$)	Product	Annealing	Usage
name	region		length	temperature
	or gene		(bp)		(${}^{\circ }$C)
P1	5${}^{\prime }$ UTR $+$ exon1	F: CTCTAAATCCCTCTTCT	949	52	SNP detection
		R: AGCAGCCCTCCCTACAA
P2	exon2	F: AGATCCCTTTGCCTGTC	805	54	SNP detection
		R: TTGGGTCTAGTGTATCCTTT
P3	exon3	F: AAAATACTTGCCTACTACAC	865	55	SNP detection
		R: TACCCAGAAACAGGATAA
P4	exon4	F: AATAGTTTCGGTATGGTAG	909	52	SNP detection
		R: ACTGCTGCTGTTACTGG
P5	3${}^{\prime }$ UTR	F: CAGTTTGACATTCCCAGTA	1073	54	SNP detection
		R: TCCATCCACCTTATTGC
P6	IGF1	F: CGGAAGACTTAAAAAAAACTAT	169	56	RT-PCR
		R: TGGGCTGGTTAAAACGTTCTGT
$\mathit{\beta }$-actin	$\mathit{\beta }$-actin	F: CCATCTATGAGGGCTACGCT	232	56	RT-PCR
		R: GCTTCTCCTTGATGTCACGG