TABLE 2.
Primers and PCR cycling conditions for antigens.
| Gene | Primers | Cycling conditions |
| Tp1 | ||
| Round 1 | F: GCTACGCGGAAATCTAGGCT R: CATCGTTTGCCAGCACTATGA | 98°C for 30 s, 40 cycles (98°C for 10 s, 56°C for 20 s, 72°C for 2.5 min), 72°C for 2 min |
| Round 2* | F: AGGGTCAAAAAAGTTTTATTA R: TTAATTTTTGAGGTAAATTTTG | 98°C for 30 s, 37 cycles (98°C for 10 s, 54°C for 20 s, 72°C for 1.5 min), 72°C for 2 min |
| Tp4 | ||
| Round 1 | F: ATACATCCCAAGGCCAAGCT R: GGAAGGGGTTGGATAGTGCT | 98°C for 30 s, 40 cycles (98°C for 10 s, 58°C for 20 s, 72°C for 2.5 min), 72°C for 2 min |
| Round 2 | F: TTACTCATCCTGCCGCTTCT R: TGACCTCCACCTCTCAACAC | 98°C for 30 s, 30 cycles (98°C for 10 s, 64°C for 20 s, 72°C for 2 min), 72°C for 2 min |
| Tp16 | ||
| Round 1 | F: TGATCTACAAGCTCGGTGGA R: GCGGGTATTCTGTGAAGGTC | 98°C for 30 s, 35 cycles (98°C for 10 s, 68 C for 20 s, 72°C for 1.5 min), 72°C for 2 min |
| Round 2 | R: AGACATGGGAAAGGGAAGCT F: CCTCCAGTGTCTTTCCGGTA | 98°C for 30 s, 32 cycles (98°C for 10 s, 56°C for 20 s, 72°C for 1.5 min), 72°C for 2 min |