FIGURE 7.

Western blot analysis of PmLEC1 protein during embryonic and vegetative growth reveals a ~59 kD form during early embryogenesis and late germination, and a ~36 kD form during late embryogenesis and early germination. Total protein was extracted from developing seeds harvested from Douglas‐fir trees at the indicated dates and ZE stages, mature imbibed seeds, mature stratified seeds, mature seeds incubated on germination medium for 2, 6, 10, 12, 14, 45 and 90 days, as well as from vegetative buds and pollen cones. The protein extracts (20 μg each) were resolved by SDS‐PAGE, transferred to a PVDF membrane and probed with PmLEC1‐specific polyclonal antibodies. DAEG: days after exposure to germination conditions