Figure 2.

IDO-1 inhibition in primary undifferentiated neurons infected by ZIKV. Primary culture of cortical-striatal neurons on D5. Neuronal culture from C57BL/6 mice were infected with ZIKV (MOI 0.1) and treated with 1-MT inhibitor at concentrations of 3; 10; 30 and 100 µM. After 48 hours, (A) the supernatant was collected to assess viral load, (B) cell death was assessed using the LIVE/DEAD Cell Viability Assay. (C) Representative images of infected and uninfected neurons stained with calcein AM (green indicates live cells) and ethidium homodimer (red indicates dead cells). All results are expressed as median and are representative of at least two independent experiments. Statistically significant differences were assessed by One-way ANOVA plus Holm-Sidak’s or Tukey’s comparisons test. (####) for P < 0,0001 compared to the MOCK group; (***) for P < 0,001 and (****) for P < 0,0001 compared to the ZIKV group.