Figure 7.

TFRC expression influenced by 5-zacytidine and overexpressing TFRC promotes proliferation, migration and invasion in Pca cells 22RV1 and LNCaP. (A) The protein levels of TFRC in Pca cells (C4-2, LNCaP, 22RV1 and PC-3) were detected by western blots. (B) 5-azacytidine inhibited DNA methylation and increased the expression of TFRC in LNCaP and 22RV1 cells. (C, D). The efficiency of TFRC overexpressed lentivirus were confirmed using fluorescence microscope and western blots. (E, F). The MTT and colony formation assays indicated that TFRC promotes cells proliferation in LNCaP and 22RV1. (G, H). Transwell assays suggested that TFRC promotes cells migration and invasion in LNCaP and 22RV1. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.