Fig. 1.

Decreased expression of MUC17 in H. pylori-infected GC. a RT-qPCR of the mRNA expression of membrane-bound mucins (MUC1, MUC12, MUC13, MUC16, MUC17, and MUC20) in GC cell BGC823 and SGC7901 after infected H. pylori at multiplicity of infection of 50 for 12 h (MOI 50, 12 h). Each gene was compared with its own untreated control after normalization to β-actin. b RT-qRCR of MUC17 expression in BGC823 and SGC7901 cells with H. pylori infection at different multiplicity of infection (MOI 5, 25, 50, 100) for 12 h. c RT-qRCR of MUC17 expression in BGC823 and SGC7901 cells with H. pylori infection (MOI 50) at different time points (0, 6, 12, 18 h). d Representative immunohistochemical staining of MUC17 expression in human GC tissues with or without H. pylori infection. Original microphotographs at magnification of × 400. Scale bar, 50 μM. e Analysis of MUC17 expression in association with H. pylori infection in the GC tissues by IHC (n = 67). *p < 0.05, **p < 0.01by χ² test. f Kaplan–Meier analysis of overall survival until 120 months for the GC patients in accordance with H. pylori infection and the MUC17 expression level. *p < 0.05, **p < 0.01. Hp: H. pylori