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. Author manuscript; available in PMC: 2021 Jul 29.
Published in final edited form as: Anat Rec (Hoboken). 2020 Apr 3;303(6):1717–1726. doi: 10.1002/ar.24393

Figure 3. Endothelial – endothelial communications demonstrated by fluorescence recovery in the central cornea after photo-bleaching.

Figure 3.

(A) Endothelial cells in the central cornea were uploaded with Calcein AM. (B) A circular area of the endothelial monolayer was photo-bleached. (C) The intensity of fluorescence recovery after photo-bleaching was evaluated at 2 different areas of the circle, center -blue circle- and outer circumference – red donut. (D) All bleached endothelial cells recover fluorescence at 100 seconds. (E) Graphic shows speed and pattern of fluorescence recovery in the central cornea. Note that endothelial cells in the periphery of the bleached circle recover fluorescence first, followed by the central endothelial cells with a similar fluorescence recovery rate demonstrated by a similar Delta between center and outer rim at 10 and 100 seconds. Note: a representative experiment is shown in this graphic. This image does not represent table 1.