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. Author manuscript; available in PMC: 2021 Jul 29.
Published in final edited form as: Anat Rec (Hoboken). 2020 Apr 3;303(6):1717–1726. doi: 10.1002/ar.24393

Figure 4. Endothelial – stromal communications demonstrated by Fluorescence Recovery in the Peripheral Cornea after photo-bleaching.

Figure 4.

(A) Endothelial cells were uploaded with Calcein AM. (B) A circular area of the endothelial monolayer was photobleached. (C) The intensity of fluorescence recovery after photobleaching was evaluated at 2 different areas of the photobleached circle. (D) Endothelial cells recovered fluorescence at 100 seconds. (E) Graphic shows speed and pattern of fluorescence recovery in the peripheral cornea. Note that endothelial cells in the periphery of the bleached circle recover fluorescence first, followed by the central endothelial cells. There is a faster fluorescence recovery rate in the center area compared to the outer rim. The 2 lines are closer at 100 seconds. A representative experiment is shown. This image does not represent table 1.