Fig 5. ETE 19–1 modulates the levels of autophagy-related proteins.

(A) Modulation of autophagy was assessed by western blot analyzing the levels of p62 and LC3-II. Cells were transfected with polyQ₁₁-EGFP, polyQ₇₉-EGFP, or mHTT_Q85-GFP expression vectors. 4 h post transfection, cells were treated with ETE 19–1 at 100 µg/mL. 16 h later, cells were collected, and total protein extracted. p62 and endogenous lipidated LC3-II levels were monitored by western blot using anti-p62 and anti-LC3 antibodies, respectively (upper and lower panel). Actin expression was monitored as loading control (middle panel). (B) p62 and (C) LC3-II levels were quantified and normalized to actin. Results are the mean ± SEM of 3 independent experiments. Statistically significant differences were detected by unpaired t-test.