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. 2021 Jul 29;16(7):e0255249. doi: 10.1371/journal.pone.0255249

Fig 1. Cytological characterization of primary culture of ovine pineal gland cells.

Fig 1

Five (A) and eight days (B) primary culture of ovine pineal glands were observed by optical microscopy. Cells from ovine pineal glands were immunolabelled with anti-tryptophan hydroxylase (TPH, C) or anti- glial fibrillary acidic protein (GFAP, D) antibodies and detected by a peroxydase-coupled secondary antibody. Non immun serum (NIS) was used as a negative control (Fig 1E). Magnification was x400 in A and C, x200 in B and x100 in D. Pinealocyte were labelled with an anti-TPH antibody (G) detected by a cyanin-coupled secondary antibody, while astrocytes were labelled with an anti-GFAP antibody (H) detected by fluorescein-coupled secondary antibody. Nuclei were stained with DAPI (F). The merge of the three labelings is shown in I. Maginifcation is x10. Scale bar 50 μm. Astrocytes (arrow).