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. 2021 Jul 29;16(7):e0255249. doi: 10.1371/journal.pone.0255249

Fig 3. Coupling of MT receptor to Gi proteins in pinealocytes.

Fig 3

(A) Effect of 10−7 M MLT on cAMP production induced by 1 μM of Forskolin (Fsk). Effect of pre-treatment with Pertussis toxin (PTX; 5 ng/ml; 3 h at 37°C) on cAMP accumulation following stimulation with 10−7 M MLT and 1 μM of Fsk (1 h at 37°C). Each histogram is presented as a percentage of the maximal response to 1 μM of forskolin (100%). B) Effect of 10−7 M MLT or of a pre-treatment with Pertussis toxin (PTX; 5 ng/ml; 3 h at 37°C) and then 10−7 M MLT (5 min) on the level of phosphorylated ErK 1/2. (C) The Western blot is a representative experiment. Columns and bars represent mean ± s.e.m. values of data obtained from duplicate determinations of cAMP in 5 different experiments (A) and 3 assays for the phospho-Erk1/2 level (B and C). *P<0.05 in Kruskal-Wallis tests followed by the Dunn’s multiple comparison tests.