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. 2021 Jul 19;17(7):e1009682. doi: 10.1371/journal.pgen.1009682

Fig 7. Round-robin duels between six LXG toxin–antitoxin deletion mutants.

Fig 7

(A) The indicated LXG toxin–antitoxin deletion mutants, with or without the gfp reporter, were co-cultured at a 1:1 ratio on MSgg solid medium. After 48 h, the proportion of GFP-reporter strains within colonies was determined as mean ± standard deviation (n = 3). Fluorescent images of colonies are also shown. The experiments were performed at least three times, and representative examples are shown in the figures. The mutant strains are arranged from left (top) to right (bottom) in order of competitiveness. Scale bar, 2 mm. (B). Enlarged images of colony centers. The values of the GFP strain (%) are from panel A. Scale bar, 0.2 mm. (C) LXG toxins function synergistically. The ΔyxiB–E mutant with the gfp reporter was co-cultured with double LXG toxin–antitoxins mutants. The proportion of GFP-reporter strains within colonies was reported as mean ± standard deviation (n = 3). Fluorescent images of colonies are also shown. Scale bar, 2 mm.