Fig 8. LXG toxins are effective against natural isolates of B. subtilis.

The GFP-labeled strain 3610 or the T7SS mutant ΔyukE–D was co-cultured with a B. subtilis natural isolate at a ratio of 1:1 on MSgg medium. After 48 h of cultivation, the morphology and GFP fluorescence of colonies were observed with a stereomicroscope. Single-strain cultures are shown as references. Selected results are shown; see S10 Fig for complete results. The experiments were repeated twice and confirmed the reproducibility. The classification of co-cultured colonies is indicated on the right. Scale bar, 2 mm.