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. 2021 Jul 19;17(7):e1009682. doi: 10.1371/journal.pgen.1009682

Table 3. B. subtilis strains used in this study.

Strains Genotypes References or constructiona
NCIB3610 prototroph [20]
N1920 amyE::PyeeF-gfp (cat) This study
N1939 amyE::PyeeF-gfp (cat) ΔdegU::kan N345 (ΔdegU::kan) [26] → N1920
N1921 amyE::PyobL-gfp (cat) This study
N1940 amyE::PyobL-gfp (cat) ΔdegU::kan N345 (ΔdegU::kan) [26] → N1921
N1922 amyE::PyokI-gfp (cat) This study
N1941 amyE::PyokI-gfp (cat) ΔdegU::kan N345 (ΔdegU::kan) [26] → N1922
N1923 amyE::PyqcG-gfp (cat) This study
N1942 amyE::PyqcG-gfp (cat) ΔdegU::kan N345 (ΔdegU::kan) [26] → N1923
N1917 amyE::PywqH-gfp (cat) This study
N1944 amyE::PywqH-gfp (cat) ΔdegU::kan N345 (ΔdegU::kan) [26] → N1917
N1918 amyE::PyxiB-gfp (cat) This study
N1943 amyE::PyxiB-gfp (cat) ΔdegU::kan N345 (ΔdegU::kan) [26] → N1918
N2302 amyE::Pspac-hy-yeeF (cat) This study
N2303 amyE::Pspac-hy-yobL (cat) This study
N2304 amyE::Pspac-hy-yokI (cat) This study
N2305 amyE::Pspac-hy-yqcG (cat) This study
N2306 amyE::Pspac-hy-ywqJ (cat) This study
N2307 amyE::Pspac-hy-yxiD (cat) This study
N2296 amyE::Pspac-hy-yeeF-yeeG (cat) This study
N2297 amyE::Pspac-hy-yobL-yobK (cat) This study
N2298 amyE::Pspac-hy-yokI-yokJ (cat) This study
N2299 amyE::Pspac-hy-yqcG-yqcF (cat) This study
N2300 amyE::Pspac-hy-ywqJ-ywqK (cat) This study
N2301 amyE::Pspac-hy-yxiD-yxxD (cat) This study
N1925 ΔyeeF-G::cat This study
N1927 ΔyobL-K::cat This study
N1926 ΔyokI-J::cat This study
N1928 ΔyqcG-F::cat This study
N1993 ΔywqH-L::cat W593 [39] →NCIB3610
N1994 ΔyxiB-yxxE::cat W592 [39] →NCIB3610
N1789 amyE::Pspac-hy-gfp (cat) This study
N1957 amyE::Pspac-hy-gfp (tet) pCM::TC [70] → amyE::Pspac-hy-gfp (cat)
N2030 ΔyeeF-G::cat amyE::Pspac-hy-gfp (tet) N1957 → N1925
N2031 ΔyobL-K::cat amyE::Pspac-hy-gfp (tet) N1957 → N1927
N1934 ΔyokI-J::cat amyE::Pspac-hy-gfp (tet) N1957 → N1926
N2033 ΔyqcG-F::cat amyE::Pspac-hy-gfp (tet) N1957 → N1928
N2034 ΔywqH-L::cat amyE::Pspac-hy-gfp (tet) N1957 → N1993
N2035 ΔyxiB-yxxE::cat amyE::Pspac-hy-gfp (tet) N1957 → N1994
N2024 ΔyeeF-G::cat amyE::Pspac-hy-yezG (erm) This study
N2025 ΔyobL-K::cat amyE::Pspac-hy-yobK (erm) This study
N2074 ΔyokI-J::cat amyE::Pspac-hy-yokJ (erm) This study
N2027 ΔyqcG-F::cat amyE::Pspac-hy-yqcF (erm) This study
N2028 ΔywqH-L::cat amyE::Pspac-hy-ywqK (erm) This study
N2029 ΔyxiB-yxxE::cat amyE::Pspac-hy-yxxD (erm) This study
N1448 ΔyukE-yueD::cat W1102 [39] →NCIB3610
N1997 ΔyukE-yueD::spc pCM::SP [70] → ΔyukE-yueD::cat
N2401 ΔyukE (in-frame deletion) This study
N2402 ΔyukC (in-frame deletion) This study
N2403 ΔyukE amyE::Pspac-hy-yukE (cat) This study
N2404 ΔyukC amyE::Pspac-hy-yukC (cat) This study
N355 ΔepsA-O::spc [71]
N2068 ΔyeeF-G::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1925
N2069 ΔyobL-K::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1927
N2070 ΔyokI-J::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1926
N2071 ΔyqcG-F::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1928
N2072 ΔywqH-L::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1993
N2073 ΔyxiB-yxxE::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1994
N11 ΔtapA-tasA::erm [71]
N2053 ΔyeeF-G::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1925
N2054 ΔyobL-K::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1927
N2055 ΔyokI-J::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1926
N2056 ΔyqcG-F::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1928
N2057 ΔywqH-L::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1993
N2058 ΔyxiB-yxxE::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1994
N364 ΔepsA-O::spc ΔtapA-tasA::erm N11 → N355
N2059 ΔyeeF-G::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N2068
N2060 ΔyobL-K::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N2069
N2061 ΔyokI-J::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N2070
N2062 ΔyqcG-F::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N2071
N2063 ΔywqH-L::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N2072
N2064 ΔyxiB-yxxE::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N2073
N1976 ΔyeeF-G::erm pCM::EM [70] → ΔyeeF-G::cat
N1977 ΔyokI-J::erm pCM::EM [70] → ΔyokI-J::cat
N1978 ΔyqcG-F::erm pCM::EM [70] → ΔyqcG-F::cat
N2018 ΔyeeF-G::erm ΔyokI-J::cat N1976 → N1926
N2019 ΔyokI-J::cat ΔyqcG-F::erm N1977 → N1926
N2020 ΔyeeF-G::erm ΔyqcG-F::cat N1976 → N1928
N2021 ΔyeeF-G::erm ΔywqH-L::cat N1976 → N1993
N2022 ΔyokI-J::erm ΔywqH-L::cat N1977 → N1993
N2023 ΔyqcG-F::erm ΔywqH-L::cat N1978 → N1993
N1998 ΔyukE-yueD::spc amyE::Pspac-hy-gfp (tet) N1957 → N1997
N2075 amyE::PwapA-gfp (cat) This study
N2076 amyE::PwapA-gfp (cat) ΔdegU::kan ΔdegU::kan [26] → N2075
N1953 ΔwapAI::cat This study
N1958 ΔwapAI::cat amyE::Pspac-hy-gfp (tet) ΔdegU::kan [26] → N1953
N2077 ΔwapAI::cat ΔepsA-O::spc amyE::Pspac-hy-gfp (tet) N355 → N1958
N2078 ΔwapAI::cat ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N11 → N1958
N2088 ΔwapAI::cat ΔepsA-O::spc ΔtapA-tasA::erm amyE::Pspac-hy-gfp (tet) N2077 → N2078