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. 2021 Mar 15;38(8):3078–3092. doi: 10.1093/molbev/msab071

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Rates of bone matrix deposition in cichlids. Mandibles of LF (A) and TRC (B) are shown, and the ascending arm of the articular bone (AP) is labeled. The tip of the AP in TRC reared in either a benthic/biting (C) and pelagic/sucking (D) environment is shown. Panels (C and D) are overlays of bright field, GFP, and RFP illumination. The RFP filter shows where alizarin red was incorporated into the bone. GFP is the calcein green label 5 weeks later. The distance between labels (white arrows) represents the amount of matrix deposited during that time. Scale bars equal 50 µm. Quantification of the rates of bone matrix deposition are shown in (E). Significance was determined via an ANOVA followed by a Tukey’s multiple comparison test.