Table 1.
Expression Differences of Bone Marker Genes.
| Osteoblast markers: | Runx2b | Osx | AP | |||
|---|---|---|---|---|---|---|
| F-value | P-value | F-value | P-value | F-value | P-value | |
| Genotype | 5.982 | 0.0163 | 2.796 | 0.0982 | 5.994 | 0.01621 |
| Age | 31.757 | 1.78E-07 | 3.23 | 0.0759 | 11.466 | 0.00104 |
| GxA | 22.254 | 8.20E-06 | 0.908 | 0.3435 | 10.043 | 0.00206 |
|
| ||||||
| Osteoclast markers: | Csf1ra | TRAP | ||||
| F-value | P-value | F-value | P-value | |||
|
| ||||||
| Genotype | 2.824 | 0.097185 | 1.567 | 0.214 | ||
| Age | 13.951 | 3.73E-04 | 2.589 | 0.111 | ||
| GxA | 1.557 | 0.216196 | 0.381 | 0.539 | ||
|
| ||||||
| Chondroblast markers: | Col10a1* | Col2a1a | ||||
| F-value | P-value | F-value | P-value | |||
|
| ||||||
| Genotype | 3.986 | 0.048744 | 0.045 | 0.8317 | ||
| Age | 12.967 | 5.06E-04 | 4.929 | 0.0291 | ||
| GxA | 0.009 | 0.925719 | 0.217 | 0.6424 | ||
|
| ||||||
| Hedgehog markers: | Ptch1 | Ptch2 | Gli | |||
| F-value | P-value | F-value | P-value | F-value | P-value | |
|
| ||||||
| Genotype | 6.758 | 0.0111 | 18.683 | 6.39E-05 | 5.373 | 0.0233 |
| Age | 6.351 | 1.37E-02 | 18.93 | 5.80E-05 | 0.845 | 0.361 |
| GxA | 1.09 | 0.2996 | 4.324 | 0.0422 | 0.04 | 0.8415 |
Note.—Expression of genes involved in bone/cartilage development was assessed in WT and mutant animals at two life-history stages, young adult (3–5 months) and old adult (10–15 months). The ANOVA model was (expression ∼ genotype × age), and the effects of genotype, age, and their interaction are presented. Marker genes are organized by general function. Col10a1 has an asterisk next to it, because it plays roles in both endochondral and dermal bone formation in fishes. Values with significance after Bonferroni-correction are italicized.