Table 1.
Reproductive Traits for Solanum habrochaites Accessions.
| Accession | Mating System and SC Group | S-RNase Protein | S-RNase Allele(s) | HT Protein | Interpop pollen-side | Interpop pistil-side | UI | RAD-Seq Cluster |
|---|---|---|---|---|---|---|---|---|
| LA4656 | SC-2 | N | LhgSRN-1 | Y | Y | N | Y | na |
| LA1624a | SC-2 | N | LhgSRN-1 | Y | Y | N | Y | C1b |
| PI129157a | SC-2 | N | LhgSRN-1 | Y | Y | N | Y | C1b |
| LA1625a | SC-2 | N | LhgSRN-1 | Y | Y | N | Y | C1 |
| LA1266a | SC-1 | N | hab-7 | Y | N | N | Y | na |
| PI134417 | SC-2 | N | LhgSRN-1 | Y | nt | N | nt | C1b |
| LA1264a | SC-1 | N | hab-7 | Y | N | N | Y | na |
| PI390515 | SC-2/3 | N | LhgSRN-1 | N | Y | N | N | C1b |
| LA0407a | SC-2 | N | LhgSRN-1 | Y | Y | N | Y | C1 |
| LA1223a | SC-3 | N | LhgSRN-1 | N | N | N | N | C1 |
| PI251305 | SC-1/2/3 | Y | hab-7/LhgSRN-1 | N | N | Y | Y | C1b |
| LA4654 | SC-6 | N | Unknown | Y | N | N | Y | na |
| LA4655 | SC-6 | N | Unknown | low | N | N | Y | na |
| LA2119a | SC-1 | N | hab-7 | Y | N | N | Y | C2 |
| LA2868a | SI | Y | Multiplec | Y | N | Y | Y | C1b |
| LA2128 | SC-1 | N | hab-7 | Y | N | N | Y | C2 |
| LA1252 | SC-1 | N | hab-7 | Y | N | N | Y | C2 |
| LA2855d | MP | Y | Multiple | Y | N | Y | Y | C3 |
| LA2106a | SC-1 | N | hab-7 | Y | N | N | Y | C2 |
| LA2101a | SC-5 | N | Unknown | Y | N | N | Y | C3b |
| LA2860 | SC-5 | N | Unknown | Y | N | N | Y | C2 |
| LA2864a | SI | Y | Multiple | Y | N | Y | Y | C3b |
| LA2099a | MP | Y | Multiplec | Y | N | Y | Y | C3b |
| LA2098a | MP | Y | Multiple | Y | N | Y | Y | C3 |
| LA2175a | MP | Y | Multiple | Y | N | Y | Y | C5 |
| LA1391a | MP | Y | Multiplec | Y | N | Y | Y | C4b |
| LA2314a | SI | Y | Multiple | Y | N | Y | Y | na |
| LA1353a,e | SI | Y | Multiplec | Y | N | Y | Y | na |
| LA1777a,e | SI | Y | Multiple | Y | N | Y | Y | C7 |
| LA0094d | MP | Y | Multiplec inc hab-6 | Y | N | Y | Y | C8 |
| LA1560d | SC-4 | Y | hab-6 | nt | N | nt | nt | C8 |
| LA1753d | SC-4 | Y | hab-6 | Y | N | nt | nt | C8 |
| LA1691 | SC-4 | Y | hab-6 | nt | N | nt | Y | C8 |
| LA1927e | SC-4 | Y | hab-6 | Y | Y | Y | Y | C8b |
Note.—Reproductive traits documented for each accession include mating system as detected by fruit production after self-pollination and/or pollen tube growth analysis (SC, self-compatible; SI, self-incompatible); expression of S-RNase protein as detected by immunoblotting; S-RNase allele as detected by allele-specific PCR. Expression of HT protein as detected by immunoblotting; pollen-side interpopulation reproductive barriers as detected by pollen tube growth in crosses with pollen from different accessions onto pistils of SI accession LA1777 (Y = pollen tubes rejected, N = pollen tubes accepted); pistil-side interpopulation barriers as detected by pollen tube growth with pollen of SC accession LA0407 onto pistils of different accessions (Y = pollen rejected, N = pollen accepted); interspecific unilateral incompatibility (UI) detected by pollen tube growth in crosses using cultivar (Solanum lycopersicum) pollen onto pistils of different accessions (Y = pollen tubes rejected, N = pollen tubes accepted); and SC group based on the combination of reproductive traits and S-RNase allele present.
Data from Broz, Randle, et al. (2017).
By inference, due to either TSS data or due to geographic location, na = not applicable because no accessions from this region were included in the RAD-seq analysis, nt = not tested. Unshaded portion of the table shows accessions from Ecuador and shaded portion of the table shows accessions from Peru.
Presence of LhgSRN-1 allele detected at a low frequency.
Mating system reported here differs from TGRC designation.
Data from Covey et al. (2010).