Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Plasmodium falciparum 3D7 strain) | Kelch13 (K13) | PlasmoDB | PF3D7_1343700 | |
| Gene (Plasmodium falciparum 3D7 strain) | Ferredoxin (fd) | PlasmoDB | PF3D7_1318100 | |
| Gene (Plasmodium falciparum 3D7 strain) | Multidrug resistance protein 2 (mdr2) | PlasmoDB | PF3D7_1447900 | |
| Strain, strain background (Plasmodium falciparum) |
3D7 clone A10 (3D7WT) | D. Goldberg, Washington University School of Medicine, St. Louis, MO, USA | see Table 1 and Supplementary file 4 for additional details on all P. falciparum strains employed herein | |
| Strain, strain background (Plasmodium falciparum) |
F32-TEM (F32WT) | F. Benoit-Vical, Université de Toulouse, Toulouse, France Ariey et al., 2014 |
||
| Strain, strain background (Plasmodium falciparum) |
UG659 (UG659WT) | P. Rosenthal, University of California, San Francisco, CA, USA | ||
| Strain, strain background (Plasmodium falciparum) |
UG815 (UG815WT) | P. Rosenthal, University of California, San Francisco, CA, USA | ||
| Strain, strain background (Plasmodium falciparum) |
Dd2 (Dd2WT) | The Malaria Research and Reference Reagent Resource Center (MR4), BEI Resources | MRA-156 | |
| Strain, strain background (Plasmodium falciparum) |
Cam3.II (Cam3.IIR539T) | R. Fairhurst, NIAID, NIH, Bethesda, MD, USA Straimer et al., 2015 |
PH0306-C | |
| Strain, strain background (Plasmodium falciparum) |
CamWT | R. Fairhurst, NIAID, NIH, Bethesda, MD, USA Straimer et al., 2015 |
PH0164-C | |
| Strain, strain background (Plasmodium falciparum) |
RF7 (RF7C580Y) |
R. Fairhurst, NIAID, NIH, Bethesda, MD, USA Ross et al., 2018 |
PH1008-C | |
| Strain, strain background (Plasmodium falciparum) |
Thai1WT | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | TA32A2A4 | |
| Strain, strain background (Plasmodium falciparum) |
Thai2WT | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | TA50A2B2 | |
| Strain, strain background (Plasmodium falciparum) |
Thai3WT | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | TA85R1 | |
| Strain, strain background (Plasmodium falciparum) |
Thai4WT | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | TA86A3 | |
| Strain, strain background (Plasmodium falciparum) |
Thai5WT | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | NHP-01334-6B | |
| Strain, strain background (Plasmodium falciparum) |
Thai6E252Q | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | NHP4076 | |
| Strain, strain background (Plasmodium falciparum) |
Thai7E252Q | T. Anderson, Texas Biomedical Research Institute, San Antonio, TX, USA | NHP4673 | |
| Strain, strain background (Escherichia coli) |
HST08 | Takara | Cat. #636766 | Stellar Competent Cells |
| Genetic reagent (Plasmodium falciparum) |
Transgenic parasite lines | This study and Straimer et al., 2015 | See Supplementary file 5 | Available from D. Fidock upon request |
| Commercial assay or kit | In-Fusion HD Cloning Plus kit | Takara | Cat. #638909 | |
| Commercial assay or kit | QuantiFast Multiplex PCR Kit | Qiagen | Cat. #204654 | |
| Sequence-based reagents | Oligonucleotides | This study | See Supplementary file 7 | |
| Recombinant DNA reagents | Plasmids | This study | See Supplementary file 8 | Available from D. Fidock upon request |
| Sequence-based reagents | qPCR primers and probes | This study | See Supplementary file 9 | |
| Antibody | Anti-K13 (P. falciparum) (Mouse monoclonal) |
I. Trakht, Columbia University Medical Center, New York, NY, USA Gnädig et al., 2020 |
Antibody clone E9 WB (1:1000) |
|
| Antibody | Anti-ERD2 (P. falciparum) (Rabbit polyclonal) |
MR4, BEI Resources | MRA-1 | WB (1:1000) |
| Antibody | StarBright Blue 700 goat anti-mouse | Bio-Rad | 12004158 | WB (1:200) |
| Antibody | StarBright Blue 520 goat anti-rabbit | Bio-Rad | 12005869 | WB (1:1000) |
| Other | 4–20% Criterion TGX Precast Protein Gel | Bio-Rad | 5671093 | Used with recommended buffers, also purchased from Bio-Rad |
| Chemical compound, drug | Carbenicillin disodium salt | Sigma | C1389 | |
| Chemical compound, drug | WR99210 | Jacobus Pharmaceuticals | ||
| Chemical compound, drug | Dihydroartemisinin (DHA) | Sigma | D7439 | |
| Software, algorithm | GraphPad Prism Version 9 | GraphPad Software, San Diego, CA, USA | graphpad.com | |
| Software, algorithm | ImageJ software | NIH, Bethesda, MD, USA | imagej.nih.gov |