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. 2021 Jul 12;10:e66396. doi: 10.7554/eLife.66396

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© 2021, Romero and Trussell

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (A) ChAT-Cre/tdTomato-positive neurons in the LSO and VNTB of the superior olivary complex co-labeled with anti-ChAT antibody (B) confirming they are cholinergic neurons (C). ChAT-Cre/tdTomato-positive neurons in the ipsilateral LSO (D) and contralateral VNTB (E) were retrogradely labeled by cochlear CTB injections. (F) Contralateral to cochlear CTB injections, 66.1% of ChAT-Cre/tdTomato-positive VNTB neurons were labeled. In ipsilateral VNTB, 28.9% of ChAT-Cre/tdTomato=positive neurons were labeled (N = 3 mice, 205 cells). Abbreviations: LNTB, lateral nucleus of the trapezoid body; LSO, lateral superior olive; VNTB, ventral nucleus of the trapezoid body; CTB, cholera toxin subunit B.