Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (M. musculus) | ChAT-IRES-Cre | Jackson Laboratory PMID:21284986 | RRID:IMSR_JAX:006410 | |
| strain, strain background (M. musculus) | Ai9(RCL-tdT) | Jackson Laboratory PMID:22446880 | RRID:IMSR_JAX:007909
|
|
| strain, strain background (M. musculus) | C57BL/6J | Jackson Laboratory | RRID:IMSR_JAX:000664 | |
| Chemical compound, drug | Strychnine hydrochloride | Sigma | Cat# S8753 | |
| Chemical compound, drug | SR-95531 hydrobromide | Tocris | Cat# 1262 | |
| Chemical compound, drug | NBQX disodium salt | Tocris | Cat# 1044 | |
| Chemical compound, drug | (+)-MK-801 hydrogen maleate | Sigma Aldrich | Cat# M107 | |
| Chemical compound, drug | Tetrodotoxin (TTX) | Sigma Aldrich | CAT# 554412 | |
| Chemical compound, drug | Biocytin | ThermoFisher Scientific | Cat# B1592 | |
| Chemical compound, drug | Cholera toxin subunit B | List Labs | Cat# 104 | |
| Antibody | anti-ChAT (Goat polyclonal) | Millipore | Cat# AB144P RRID:AB_2079751 |
IHC (1:500) |
| Antibody |
anti-GFP (Chicken polyclonal) | Aves Labs | Cat# GFP-1020RRID:AB_10000240 | IHC (1:1000) |
| Antibody | anti-Cholera Toxin B Subunit (Goat polyclonal) | List Labs | Cat# 703 RRID:AB_10013220 | IHC (1:1000) |
| Antibody | anti-DsRed (Rabbit polyclonal) | Clontech | Cat# 632496RRID:AB_10013483 | IHC (1:500) |
| Antibody | anti-chicken Alexa Fluor 488 (Donkey polyclonal) | Jackson ImmunoResearch Labs | Cat# 703-545-155RRID:AB_2340375 | IHC(1:1000) |
| Antibody | anti-goat Alexa Fluor 488 (Donkey polyclonal) | Jackson ImmunoResearch Labs | Cat# 705-545-147 RRID:AB_2336933 | IHC (1:500) |
| Antibody | anti-Rabbit Alexa Fluor 594 (Donkey polyclonal) | Jackson ImmunoResearch Labs | Cat# 711-585-152RRID:AB_2340621 | IHC(1:500) |
| Antibody | Streptavidin-Alexa Fluor 647 | ThermoFisher Scientific | Cat# S21374RRID:AB_2336066 | IHC (1:2000) |
| Recombinant DNA reagent | AAVrg-pmSyn1-EBFP-Cre (6×10¹² GC/mL) | addgene | Cat# 51507-AAVrg | |
| Recombinant DNA reagent |
AAV9-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-HGHpA (2.2×1013 GC/mL) | addgene | Cat# 35507-AAVrg | |
| Recombinant DNA reagent |
AAV1-CAG-ChR2-Venus-WPRE-SV40 (8.99×1012 GC/mL) | addgene | Cat# 20071-AAV1 | |
| Recombinant DNA reagent |
AAV2-EF1a-DIO-hChR2(E123T/T159C)-p2A-eYFP-WPRE | addgene | Cat# 35509-AAV2 | |
| Software, algorithm | pClamp 10 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | Igor Pro 8 | WaveMetrics | RRID:SCR_000325 | |
| Software, algorithm | NeuroMatic | Rothman and Silver, 2018; DOI:10.3389/fninf.2018.00014 | RRID:SCR_004186 | |
| Software, algorithm | Axograph | Axograph | RRID:SCR_014284 | |
| software, algorithm | Excel | Microsoft |
RRID:SCR_016137 | |
| Software, algorithm | FIJI | Fiji.sc | RRID:SCR_002285 | |
| Software, algorithm | Adobe Illustrator CS2 | Adobe | RRID:SCR_010279 |