Skip to main content
. 2021 Jul 14;10:e67113. doi: 10.7554/eLife.67113

Figure 9. Identification of likely sources of Tn916-type and Tn1207.1-type elements.

Flanking regions upstream and downstream from mobile genetic element (MGE) insertion sites were compared to a reference streptococcal database. Lines represent the proportion of matches, across all element acquisitions reconstructed as having occurred through homologous recombination, that correspond to the four species present in the reference streptococcal database. The dashed unmodified locus lines represent data from the orthologous regions of isolates without the MGE insertion. These proportions were calculated at 500 bp increments over 7.5 kb-long flanking regions either side of the insertion site.

Figure 9—source data 1. Closest species match to the 500 bp region upstream of tag-disrupting Tn1207.1 insertions.
The species, ENA accession codes, and frequencies of the matches to the 500 bp region upstream of the 66 Tn1207.1-type element insertions into tag that were reconstructed as having occurred within inferred homologous recombination events.

Figure 9.

Figure 9—figure supplement 1. Flanking region origin for Tn1207.1-type element tag insertions.

Figure 9—figure supplement 1.

(A) The median γ score of upstream flanking regions of the Tn1207.1-type element insertions into the tag gene, highlighted in coral. The median γ score for the orthologous regions in isolates without the mobile genetic element (MGE), not expected to be modified by homologous recombination, are highlighted in cyan. Shaded regions represent the interquartile range (IQR) of the γ score. (B) The γ score for regions extracted downstream of the insertion. Shaded regions represent the IQR of the γ score. (C) The distribution of γ scores across both flanks, upstream and downstream, of the Tn1207.1-type element tag insertions. Data are shown separately for isolates with this particular insert, and those without Tn1207.1-type elements integrated in this orthologous region.