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. 2021 Aug 2;131(15):e146323. doi: 10.1172/JCI146323

Figure 4. LAMB1 deficiency in the ACC induces apical dendritic spine remodeling of ACC pyramidal neurons.

Figure 4

(A) Representative images of pyramidal neurons in the ACC derived from sham- and SNI-treated mice expressing scrambled shRNA or shLamb1 (n = 24–30). Scale bars: 100 μm. (B and C) Sholl analysis of dendritic branching complexity in the basal and apical dendrites of sham- or SNI-operated mice expressing scrambled shRNA or shLamb1 (n = 24–30). *P < 0.05, by Kruskal-Wallis H test with Nemenyi’s multiple-comparison test. (D) Representative confocal stack and 3D reconstruction images of apical dendrites of ACC pyramidal neurons obtained from mice expressing shLamb1 or scrambled shRNA in both sham and SNI conditions (n = 20–25). Scale bars: 5 μm. (E and F) Summary of spine density (E) and length (F) of apical dendrites of ACC pyramidal neurons obtained from mice expressing shLamb1 or scrambled shRNA in both sham and SNI conditions (n = 20–25). **P < 0.01 and ****P < 0.0001, by Kruskal-Wallis H test with Nemenyi’s multiple-comparison test. (G) Summary of the density of stubby-, mushroom-, long/thin-, and filopodia-shaped spines on apical dendrites of ACC pyramidal neurons from mice of the above 4 groups (n = 22–29). ****P < 0.0001, by Kruskal-Wallis H test with Nemenyi’s multiple-comparison test. Data are presented as the mean ± SEM. See Supplemental Table 2 for detailed statistical information.