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. 2021 Jun 18;4(8):e202000910. doi: 10.26508/lsa.202000910

Figure 6. Formation of MVB-intracellular enveloped virion (IEVs) is dependent on endosomal sorting complexes required for transport machinery.

Figure 6.

(A) Representative EM images of VACV-infected HeLa cells (8 hpi) showing cis-wrapping (asterisk), and MVB-IEVs (arrowheads). Scale bars = 500 nm. (B) Quantification of the IEV classes. Data are representative of six biological replicates. Data are mean ± SEM, representative of n = 80–100 counted viral particles per replicate. (C) Representative images of VACV-infected HeLa cell cryosections (8 hpi) where EV membrane protein B5 is immunolabelled with gold. Cisternae wrapped virions (asterisks) and MVB-IEVs (arrowheads). Scale bars = 500 nm. (D, F) Representative EM images of intracellular mature virion and MVB-IEV, and quantifications of proportions of these classes. Data are representative of three biological replicates. Data are mean with min and max values. Statistical analysis was performed using unpaired two-tailed t tests (*P < 0.05; **P < 0.01; ****P < 0.0001).