Figure S1. Cell-to-cell variability in P3F expression in RMS.

(A) The mouse RMS cell line U23674 was sorted into YFP^high/P3F^high and YFP^low/P3F^low cells (purity > 98%). C2C12 cells served as YFP^neg control cells and Kras;p16p19^null mouse RMS cells as YFP^pos control cells. (B) Single-cell P3F and GAPDH expression by RT-ddPCR profiling. mRNA content is depicted for 83 GAPDH-expressing IC-pPDX 35 cells, 79 GAPDH-expressing RMSZH003 cells, 87 GAPDH-expressing SJRHB013759_X1 cells, 85 GAPDH-expressing Rh41 cells, and 46 GAPDH-expressing Rh30 cells. Each dot represents the mRNA content of one GAPDH-expressing cell.