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. 2021 Jun 29;4(9):e202001002. doi: 10.26508/lsa.202001002

Figure S6. Lower P3F dose did not change the proportion of apoptotic cells in human Rh30 and Rh5 cells RMS cells.

Figure S6.

(A) Silencing of P3F in U23674 cells by siGFP correlates with marked reduction in the percentage of YFPhigh cells. C2C12 cells served as YFPneg control cells and Kras;p16p19null mouse RMS cells as YFPpos control cells. (B) P3F silencing in Rh30 cells exposed to si-P3F compared with control cells and cells exposed to scramble siRNA. (C) Reduced P3F expression in siP3F Rh30 correlated with lower proliferation rates than in control and scramble cells. (D) Levels of cleaved Caspase 3 (Cl-Casp 3) and cleaved PARP (Cl-PARP) were similar among control, scramble and si-P3F Rh30 cells. (E) P3F silencing in Rh5 human RMS cells exposed to si-P3F compared with control cells and cells exposed to scramble siRNA. (F) Reduced P3F expression in siP3F Rh5 cells correlated with lower proliferation rates than in control and scramble cells. (G) Levels of cleaved Caspase 3 (Cl-Casp 3) and cleaved PARP (Cl-PARP) were similar among control, scramble, and si-P3F Rh5 cells. Etoposide-treated Rh30 and Rh5 cells were included as positive controls. Experiments were replicated three times.