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. 2021 Jun 14;4(8):e202001000. doi: 10.26508/lsa.202001000

Figure 1. Tissue-protective role for IL-13 during acute lung injury.

Figure 1.

WT and Il13−/− mice were infected with Nippostrongylus brasiliensis (Nb). (A) On day 2 post-infection (D2pi) and D6pi, Il13 mRNA levels were measured in the lungs of WT mice by quantitative real-time PCR (data normalised against housekeeping gene Rpl13a). (B) On D2pi, BAL fluid absorbance at 540 nm was quantified to measure airway haemorrhage. On D6pi, lung lobe sections were stained with Prussian blue and haemosiderin-laden cells (blue) were enumerated per area of tissue (scale bar = 100 µm). (C) To measure airway damage on D2pi and D6pi, lacunarity for whole lung lobes was computed. (D) Representative haematoxylin and eosin images of infected WT and Il13−/− lungs showing alveolar damage in the tissue (scale bar = 200 µm). (E) Nb-specific actin mRNA in lung tissue was measured on D2pi by quantitative real-time PCR (data normalised against housekeeping gene Rpl13a). Data (mean ± SEM) were pooled from three individual experiments with three to six mice per group (per experiment). NS not significant, *P < 0.05, ****P < 0.0001 (one-way ANOVA and Tukey–Kramer post hoc test).