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. 2021 Jun 24;4(8):e202101102. doi: 10.26508/lsa.202101102

Figure S2. Effect of DNA replication inhibition on H3.1/2 nuclear deposition in one- and two-cell embryos.

Figure S2.

(A) Embryos were treated with aphidicolin [Aphi (+)] or DMSO [Aphi (−)] at 15 h post-insemination (hpi) to inhibit the second round of DNA replication, then collected at 15 and 26 hpi for immunostaining. Two independent experiments were performed; 27 embryos in total were analyzed for each experimental group. Representative images for each condition are shown. Scale bar, 10 μm. (B) Embryos were treated with aphidicolin [Aphi (+)] or DMSO [Aphi (−)] at 5 hpi. One-cell embryos were collected at 5 hpi and 11 hpi for immunostaining to analyze the effect of DNA replication on H3.1/H3.2 nuclear localization. Three independent experiments were performed. 7–16 embryos were observed for each condition; 32–40 embryos were observed in total. Maternal and paternal pronuclei are indicated as ♀ and ♂, respectively. The laser power of confocal laser scanning microscope was increased here compared with the observation in (A). Scale bar, 10 μm.