Figure S5. Effects of H3.1 and H3.2 overexpression on the development of parthenogenetic embryos.

(A) Developmental rates of noninjected, GFP-, H3.1-, H3.2-, and H3.3-overexpressing parthenogenetic embryos (OE). Six independent experiments were performed and 101–117 embryos were observed in each experimental group. For H3.1-OE and H3.2-OE, a χ² test or Fisher’s exact test was performed and no significant difference was detected; the results were considered significant if P < 0.01 for all other conditions (noninjected, GFP-OE, and H3.3-OE). For H3.3-OE, a χ² test or Fisher’s exact test (when there was a group in which the value was below 5) was performed; the results were considered significant if P < 0.01 for both noninjected and GFP-OE. (B) Comparison of nuclear deposition of H3.1/2 between in vitro fertilized and parthenogenetic (Partheno.) embryos in which H3.2 was overexpressed. Three independent experiments were performed; 38–41 embryos were analyzed in total for each group. Scale bar, 10 μm.