Figure 7. Human pancreatic malignant tissue mechanistic analysis.

(Ai–ii) Representative hematoxylin and eosin–stained photomicrographs show induced inflammatory cells, merged ductal cells, PanIN formation, and loss of acinar cells compared with the presence of normal acinar cells, ductal cells, and islet cells in healthy pancreas tissue sections (Ai–ii). A representative photomicrograph shows induced NLRP3 (green arrows) in the accumulated macrophages (white arrows) compared with healthy pancreas tissue (Bi-ii). (Ci–ii, Di–ii) Detection of intact eosinophils (black arrow) and degranulated extracellular granular proteins (arrow heads) by anti-EPX (Ci–ii) and anti-MBP (Di–ii) in PDAC patient biopsy compared with few eosinophils in normal biopsies. Morphometric quantification of CD163⁺, NLRP3⁺, EPX⁺, and MBP⁺ cells, expressed as cells/mm² (E). (F) IL-18 levels in human PDAC compared with the normal (F). (G) Levels of NLRP3, TGF-β, fibronectin, KRAS, and p53 in human PDAC compared with the control individuals (G). Data are presented as means ± SD, *P < 0.05, **P < 0.001, ***P < 0.0001. *Represents pancreatic cancer versus control individuals, n = 6–8 human tissues/group. All photomicrographs shown are 400× (scale bar 20 µm) the original magnification.