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. 2021 Jul 21;4(9):e202101036. doi: 10.26508/lsa.202101036

Figure S4. HBV cccDNA and pgRNA quantification in tumor samples from P1 and P2.

Figure S4.

(A). Total DNA was extracted from tumor tissue from P1 and P2 patients as well as healthy primary hepatocytes from a HBV-negative control patient (Healthy Ctrl). cccDNA levels were assessed by qPCR. Results are expressed as mean ± SD cccDNA copies/μl from three independent biological samples (n = 3). (B) Total RNA was extracted from tumor tissue from P1 and P2 patients as well as healthy primary hepatocytes from a HBV-negative control patient (Healthy Ctrl). cccDNA levels were assessed by qPCR. Results are expressed as mean ± SD pgRNA mRAN/GAPDH mRNA from three independent biological samples (n = 3).