Figure S1. Functional effects of HOTAIR knockdown in HeLa cells.

(A) HOTAIR expression in multiple cell lines as measured by qRT-PCR. (B) Relative HOTAIR expression in HeLa cells after either transient transfection using siHOTAIR (siHO1 or siHO2) or siNC, or stable transfection with shHOTAIR (shHO1 and shHO2) or shNC. (C) Results of a CCK-8 assay in HeLa cells after stable or transient HOTAIR knockdown. (D) Results of a colony formation assay in HeLa cells following stable or transient HOTAIR knockdown, with the number of clones being indicated. (E) HOTAIR transient knockdown HeLa cells (siHO group) or HOTAIR stable knockdown HeLa cells (shHO group) were injected subcutaneously near the axillary fossa of nude mice. HeLa cells transfected with siNC (siNC group), or shNC expressed HeLa cells (shNC) were also injected as negative controls. The mice were euthanized after 16 d, and the tumors were dissected. (E, F) Tumor volumes from mice in (E). (E, G) Tumor weights from mice in (E). (H) HOTAIR expression in tumor samples as measured by qRT-PCR. (I) Left: Wound-healing assay in HOTAIR transiently knockdown HeLa cells. Quantification of the wound width is shown. Right: Wound-healing assay in HOTAIR stably knockdown HeLa cells. Quantification of the wound width is shown. (J) The effect of transient (top) and stably (bottom) HOTAIR knockdown on cell invasion was determined with a Boyden chamber assay. Scale bars: 60 μm. (K) The apoptosis rate of HOTAIR transient (left) and stable (right) knockdown HeLa cells. (A, B, C, D, I, K) In (A, B, C, D, I, K), data are means ± SD of three independent experiments. (E, F, G) In (EF, G), data are means ± SD (n = 8). *P < 0.05 and **P < 0.01 (t test). Fig S1 is related to Fig 1.