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. 2021 Jun 29;4(9):e202101095. doi: 10.26508/lsa.202101095

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© 2021 Nishiyama et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 5. — Clinical significances of RhoB and connector enhancer of kinase suppressor of Ras1 (CNKSR1) in human HER2-positive breast cancers. (A) Western blots of human breast cancer cell lines. MCF-7 cells (luminal type), MDA-MB-231 cells (basal type), SKBR-3 cells, and MDA-MB-453 cells (HER2-positive). (B, C, D, E, F, G) Kaplan–Meier plot with epidermal growth factor receptor (EGFR) (B, C) or RhoB (D, E, F, G) expression profiles in Molecular Taxonomy of Breast Cancer International Consortium cohort using overall survival status. Shading along the curve showed 95% confidential interval. (B) Analysis with all samples showed that high expression of EGFR was not correlated to poor prognosis (B; high: n = 974, low: n = 930). (C) In HER2-positive subtype, high expression of EGFR was correlated to poor prognosis (C; high: n = 198, low: n = 22). (D) In HER2-positive/EGFR^high type, low mRNA expression of RhoB was correlated to poor prognosis (D; high: n = 54, low: n = 30). (E) In HER2-positive/EGFR^low type, high mRNA expression of RhoB was correlated to poor prognosis (E; high: n = 14, low: n = 96). (F, G) In both HER2^high and HER2^high/CNKSR1^high breast cancers, low mRNA expression of RhoB was correlated to poor prognosis (F; High: n = 172, Low: n = 304, G; high: n = 92, low: n = 146, respectively). (H) Expression of CNKSR1 protein in human HER2-positive breast cancer tissues (n = 20). Representative images of the hematoxylin and eosin staining and immunohistochemical staining for CNKSR1 in primary lesions of HER2-positive breast cancers with metastatic recurrence were shown. The neighboring sections of the human HER2-positive breast cancer tissues were subjected to hematoxylin and eosin staining and immunohistochemistry. Bars; 100 μm. See also Table 1. (I) The Kaplan–Meier plot with protein expression profiles of CNKSR1 in human HER2-positive breast cancer using end point disease-free survival status. The high expression of CNKSR1 was correlated to poor prognosis for disease-free survival (high: n = 16, low: n = 4).

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