Table 1.

Reaction system and conditions.

Reaction system	(50 μl)	Conditions
Template DNA	5 μl	96°C	5 min
Forward primer	1 μl	95°C	20 s	13 cycles
Reverse primer	1 μl	78°C	40 s
Premix Taq	25 μl	95°C	10 s
ddH2O	18 μl	62°C	10 s	25 cycles
Total	50 μl	72°C	2 min
		72°C	5 min
		4°C	Termination reaction