Figure 6.

Role of Nrf2 on ETEC K88-induced damage promoted by CB. (a) IPEC-J2 cells incubated with Nrf2-specific siRNA (siRNA) and Nrf2 nonspecific siRNA control (NC) for 6 h. After pretreatment with CB for 3 h, the cells were treated with 1 × 10³ cfu/mL ETEC K88 for 3 h, 6 h, 12 h, and 24 h. The contents of Nrf2 were determined by qPCR. (b) After pretreatment with CB for 3 h, the cells were treated with 10³ cfu/mL ETEC K88 for 12 h. Determination of Nrf2 proteins content by Western blot. The results were mean ± SEM of three independent preparations. Value columns with different lowercase letters mean P < 0.05, while with different capital letters mean P < 0.01.