Figure 5.

Adiponectin and IL-10 are not responsible for the immunomodulatory effects of adipocytes upon airway epithelial cells, whereas IL-6 controls secretion of MIP-2. Mouse-transformed club cells (MTCCs) were treated with recombinant adiponectin and analyzed for proinflammatory cytokine secretion (A) and gene expression of Clca1 (B). MTCCs were treated with recombinant IL-10 and analyzed for cytokine secretion (C) and gene expression of Clca1 (D). In separate experiments, MTCCs were treated with lean and obese adipocyte-conditioned media (Ad-CM) in the presence or absence of anti-IL-10 neutralizing antibody, and analyzed for proinflammatory cytokine secretion (E) and Clca1 expression (F). MTCCs were treated with recombinant IL-6 and analyzed for cytokine secretion (G) and gene expression of Clca1 (H). In separate experiments, MTCCs were treated with lean and obese Ad-CM in the presence or absence of anti-IL-6 neutralizing antibody, and cytokine secretion was analyzed by ELISA (I). In separate experiments, MTCCs were treated with cytokine cocktails representative of their content as measured in LFD or HFD CM and analyzed for proinflammatory cytokine secretions (J). n = 3–8 samples/group. Data were analyzed by unpaired t test (A–D and G–I), 2-way ANOVA and Sidak’s multiple comparison test (E and F), or 1-way ANOVA and Dunnett’s multiple comparison test J). *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001.