Figure 9.

Effect of ACTH on human AZG K_IR. A: K_IR current. K⁺ currents were recorded in response to voltage steps to −120 mV applied at 30 s intervals from a holding potential of −60 mV. K_IR current amplitudes are plotted against time. Numbers on plot correspond to those on current traces in the absence and presence of 1 nM ACTH. B: combined K2P and K_IR currents. K⁺ currents were recorded in response to voltage steps to test potentials between +30 mV and −110 mV in 10 mV increments at 30 s intervals from a holding potential of −60 mV. Current amplitudes at test potentials in the absence and presence of 500 pM ACTH are plotted against time at right. Number of independent experiments: n = 3 (A and B).