Figure 10.

Effect of forskolin on human AZG K⁺ currents. A: inhibition of TREK-1. K⁺ currents were recorded in response to voltage steps to +20 mV applied at 30 s intervals from a holding potential of −80 mV. TREK-1 amplitudes are plotted against time. Numbers on plot correspond to those on traces before and after 5 µM forskolin. B: inhibition of TREK-1 and K_IR by forskolin. K⁺ currents were recorded in response to voltage steps to test potentials from +40 to −120 mV applied at 30 s intervals from a holding potential of −60 mV. Traces show IVs in control saline and in 5 µM forskolin. C: inhibited K⁺ currents and I-Vs. Difference current traces obtained by digital subtraction of K⁺ currents in forskolin from control currents. Current amplitudes from B are plotted against test potential. Number of independent experiments: n = 6 (A); n = 2 (B and C).