Table 1.
Information and references of antibodies used for Western blots
| Antibody | Species | Dilution for Western Blot | Source |
|---|---|---|---|
| pT53-NCC (Cat. No. p1311-53) | Rabbit | 1:3,000 | PhosphoSolutions (14) |
| NCC (Cat. No. AB 3553) | Rabbit | 1:2,000 | Millipore (14) |
| ENaCα (Cat. No. SPC-4030) | Rabbit | 1:1,000 | StressMarq (24) |
| ENaCβ (Cat. No. SPC-4040) | Rabbit | 1:1,000 | StressMarq (24) |
| ENaCγ (Cat. No. SPC-4050) | Rabbit | 1:1,000 | StressMarq (24) |
| Nedd4-2 (Cat. No. 40135) | Rabbit | 1:1,000 | Cell Signaling (14) |
| Actin (Cat. No. 4970 L) | Rabbit | 1:1,000 | Cell Signaling (22) |
| GAPDH (Cat. No. 2118 L) | Rabbit | 1:1,000 | Cell Signaling (22) |
ENaC, epithelial Na+ channel; NCC, NaCl cotransporter; Nedd4-2, neural precursor cell expressed developmentally downregulated 4-2; pT53-NCC, phospho-Thr53 NaCl cotransporter.