Figure 3.

Primary human fibroblasts produce hepatocyte growth factor (HGF) in response to serum or heparin. A: ELISA measurement of human HGF production by Wi-38 primary fibroblasts as a function of time and FBS concentration. Measurements were taken at 24-h intervals from triplicate wells for each sample. B: 72-h ELISA measurements of human HGF secreted by Wi-38 fibroblasts under the indicated culture conditions. Cells were grown on standard tissue culture plastic (uncoated) or on culture plates that were precoated with either a synthetic (MaxGel) or biological [Engelbreth-Holm-Swarm (EHS)] extracellular matrix (ECM). Heparin was used at 1.0 mg/mL. Basal media, RPMI-1640. C: ELISA measurements of human HGF produced by Wi-38 primary fibroblasts as a function of time and serially diluted capmatinib concentration. D: slope of the linear plots in C providing the production rate for HGF by Wi-38 fibroblasts. For all A−D, error bars represent the standard deviation of triplicate measurements. Significance was determined for each set of samples relative to the SFM or vehicle (DMSO) condition at the same time point (*P < 0.05). SFM, serum-free media; SIT, selenium-insulin-transferrin supplement.