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Example traces demonstrating loss of lumen diameter in a pressurized vessel with a leak. Representative traces showing (A) lumen diameter is stable in vessel without a leak and (B) is reduced in a preparation with a leak (either due to a branch/hole in the vessel or it was not tied to the cannulas correctly) in preparations pressurized to 100 mmHg and closed to external pressure. Lumen diameter is restored in the leaky vessel once pressure is reintroduced.
