Table 2.
Concentrations of compounds and that make up the bicarbonate buffer, the HEPES buffer, and the calcium (Ca2+)-free bicarbonate buffer
| Compound (Mol wt), g/Mol | Bicarbonate Buffer, mM | HEPES Buffer, mM | Ca2+-Free Bicarbonate Buffer, mM |
|---|---|---|---|
| NaCl (58.44) | 130.0 | 134.0 | 130.0 |
| KCl (74.55) | 5.4 | 6.0 | 5.4 |
| NaH2PO4 (119.98) | 0.5 | - | 0.5 |
| MgSO4 (246.28) | 0.8 | - | 0.8 |
| MgCl2 (95.21) | - | 1.0 | - |
| NaHCO3 (84.00) | 19.0 | - | 19.0 |
| Glucose (180.16) | 5.5 | 10.0 | 5.5 |
| EDTA (292.15) | - | 0.03 | 4.8 |
| CaCl2 (147.02) | 1.8 | 2.0 | - |
| HEPES (238.30) | - | 10.0 | - |