Figure 7.

Activity patterns—XAMB (A), XTOT (B), and ZTOT (C)—in male and female, wild-type (WT), and myoglobin knockout (Mb-KO) mice at different ambient temperatures. The results were calculated from two light and dark cycles per temperature, following an initial 24-h acclimation within each temperature (see METHODS and Fig. 1). Ages indicate approximate weeks when animals were tested under the 9 d CLAMS protocol. Three-way repeated measures ANOVA assessed differences between genotype (Geno), sex, temperature (Temp), and interactions. The interactions and P values displayed on the top of the graphs are from a three-way repeated measures ANOVA testing for effects of genotype (Geno), sex, temperature (Temp), and interactions. The P values displayed within the graphs are from a two-way repeated measures ANOVA testing for Geno and Temp effects for each sex independently, and using a Tukey’s post hoc test. *Significant differences for the temperature main effect, at P < 0.05. Values are means ± SE; males, n = 7–8 per group; females, n = 7–8 per group [due to outlier analysis (Rout test)]. XAMB, X dimension ambulatory activity; XTOT, total X dimension beam breaks; ZTOT, Total movements detected in the Z dimension.