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. 2021 May 17;321(1):E105–E121. doi: 10.1152/ajpendo.00070.2021

Table 2.

Primer sequences for quantitative RT-PCR of adipose tissue

Gene Primer 1 Primer 2
B2M CCA GCG TAC TCC AAA GAT TCA TGG ATG AAA CCC AGA CAC ATA G
CD68 CCA TGT AGC TCA GGT AGA CAA C CCA CCT GCT TCT CTC ATT CC
CD163 CCA GAA CAC ATA TTC CCT CCA C GCA TAG TAA CTG TAC TCA CCA ACA
CD206 TCC ATC TTC CTT GTG TCA GC GGT TTT GGA GTA ATA TTC ACT GTT CT
ADGRE CAT CTT CCA CCC TTC ACA TCA ACG GCT TCT CAG ATC CAA TC
MCP1 GGC TGA GAC TAA CCC AGA AAC GAA TGA AGG TGG CTG CTA TGA
INOS GCA GCT CAG CCT GTA CT CAC CAT CCT CTT TGC GAC A
IL6 GGA GAC TTG CCT GGT GAA A CTG GCT TGT TCC TCA CTA CTC
IL8 CTT GGC AGC CTT CCT GAT TT GGG TGG AAA GGT TTG GAG TAT G
TNFα GGC TGA GAC TAA CCC AGA AAC GAA TGA AGG TGG CTG CTA TGA
LEPD (LEPD) AGA GTG GCT TAG AGG AGT CAG TGG CTT CCA GGT ATC TCC A
ADIPOQ GCA GGG GAA GCA GGA CT TGC AGT CTG TGG TTC TGA TTC

ADGRE, adhesion G protein-coupled receptor E1; ADIPOQ, adiponectin; B2M, beta-2-microglobulin; CD, cluster of differentiation; IL, interleukin; INOS, inducible nitric oxide synthase; IL, interleukin; LEPD, leptin; MCP1, monocyte chemoattractant protein 1; TNFα, tumor necrosis factor-α.