Furin Inhibitor II inhibits the activation of β1-integrins and integrin-associated adhesion proteins by acetylcholine (Ach) in airway smooth muscle tissues. A: representative immunoblots from extracts of inhibitor treated or untreated muscle tissues stimulated with ACh or unstimulated (US). ACh stimulation resulted in a marked increase in the activation of β1-integrin (n = 8, P = 0.0001) and incubation with Furin Inhibitor II inhibited the increase of activated β1-integrin induced by ACh (n = 8, P = 0.0001). B: representative immunoblots from extracts of untreated or inhibitor-treated muscle tissues stimulated with ACh or unstimulated (US). The increases in vinculin Tyr1065 phosphorylation and paxillin Tyr118 phosphorylation in response to 10−5 M ACh were significantly inhibited in tissues treated with Furin Inhibitor II (vinculin: n = 7, P = 0.0001; paxillin: n = 8, P = 0.0001). Data were analyzed by using one-way ANOVA. All values are means ± SE. *Significant difference between groups. Inh, Furin Inhibitor II.