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. 2021 Jun 23;40(30):4930–4940. doi: 10.1038/s41388-021-01899-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for NADP/NADPH (left), GSH (middle), and GSH/GSSG (right) levels. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1). B DU-145/CshRNA and DU-145/PBRM1shRNA cells were analyzed for NADP/NADPH (left), GSH (middle), and GSH/GSSG (right) levels. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for CshRNA cells (assigned a value of 1). C DU-145/tet-CshRNA and DU-145/tet-MUC1shRNA cells were treated with DOX for 7 days and then incubated in the absence and presence of 12 μM H₂0₂ for 24 h. Lysates were immunoblotted with antibodies against the indicated proteins. D DU-145/CshRNA and DU-145/PBRM1shRNA cells were incubated in the absence and presence of 12 μM H₂0₂ for 24 h. Lysates were immunoblotted with antibodies against the indicated proteins. E DU-145/tet-MUC1shRNA cells were treated with vehicle or DOX for 7 days and then incubated in the presence of the indicated H₂0₂ concentrations for 24 h. F DU-145/CshRNA and DU-145/PBRM1shRNA cells were incubated in the presence of the indicated H₂0₂ concentrations for 24 h. G DU-145/tet-MUC1shRNA cells were treated with vehicle or DOX for 7 days and then incubated in the presence of the indicated concentrations of docetaxel for 48 h. H DU-145/CshRNA and DU-145/PBRM1shRNA cells were incubated in the presence of the indicated concentrations of docetaxel for 48 h. Cell viability was determined by Alamar blue staining. The results (mean ± SD of 6 determinations) are expressed as relative cell viability compared to that obtained for untreated cells (assigned a value of 1).