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. 2021 Jun 23;40(30):4884–4893. doi: 10.1038/s41388-021-01864-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A, B TSPAN8 expression was examined in the indicated cell lines by immunoblot analysis (A) or qPCR (B). C–D A wound-healing assay was performed on BxPC-3 and AsPC-1 cells expressing the control shRNA, TSPAN8 shRNA vector or reconstituted with shRNA-resistant TSPAN8. E–F Boyden chamber Matrigel invasion assays were performed on BxPC-3 and AsPC-1 cells expressing the control shRNA, TSPAN8 shRNA vector or reconstituted with shRNA-resistant TSPAN8. Scale bars: 100 μm. A, B, D and F the experiments were performed in triplicate, and the t test was performed. The values are presented as the means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.