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. 2021 Jun 23;40(30):4884–4893. doi: 10.1038/s41388-021-01864-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A 293 T cells with transient expression of Flag-SOX9 and sites in the TSPAN8 promoter were cultured for 48 h for a luciferase activity assay. B Wild-type (wt) and mutant (mut) binding sites for SOX9 in the TSPAN8 promoter region. C A luciferase activity assay was performed in 293 T cells with transient expression of the wt or mut TSPAN8 gene promoter and Flag-SOX9. D ChIP-qPCR analysis was performed. The Y axis shows the value normalized to the input. E EMSA was performed with Flag-SOX9 and probes for the site 1, site 2, mut 1 or mut 2 region of the TSPAN8 promoter. A, C and D, the experiments were performed in triplicate, and the t test was performed. The values are presented as the means ± SDs. **P < 0.01, ***P < 0.001 and ****P < 0.0001.