Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 12;18(8):1981–1994. doi: 10.1038/s41423-020-00564-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — qPCR analysis of Il6, Tnfa, and Il12b expression in control (shCtrl) and Trim60 shRNA-expressing (Trim60-shRNA #3, sh3; Trim60-shRNA #5, sh5) RAW cells stimulated with either LPS (a) or CpG (b) for the indicated amounts of time. c, d ELISA analysis of proinflammatory cytokine production in control and TRIM60-knockdown RAW cells stimulated with LPS or CpG. The indicated cells were left untreated or stimulated with either LPS (c) or CpG (d) for 12 h before ELISA analysis of IL-6 and TNFα production. e, f qPCR analysis of proinflammatory cytokine expression in BMDMs. Wild-type (WT) and TRIM60-deficient BMDMs were stimulated with either LPS (e) or CpG (f) for the indicated time periods and then subjected to qPCR analysis of proinflammatory cytokine expression. The data in a, b, e, and f are presented relative to the 18S rRNA expression level. The data in a–f are shown as the mean ± SEM and are representative of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. no significance (two-way ANOVA followed by Tukey’s multiple comparisons)