Fig. 5.

Therapeutic effects of 4-1BB CAR-T cell treatment. Mice that received low-dose TBI as preconditioning and adoptive transfer with 4-1BB CAR-T cells or PBS at 13 weeks of age were sacrificed at 22 weeks of age for subsequent examinations. a The survival rate of the mice; the difference in survival between the two groups was compared by logrank (Mantel–Cox test) calculation. b Total body weight and spleen weight. Sera were isolated from MRL-lpr mice; anti-dsDNA antibody (c) and ANA (d) levels were detected by ELISA. e Urine protein to creatinine ratios were analyzed by a chemistry analyzer. f Representative H&E-stained sections of glomerular areas of the kidneys. Black arrow, immune complex formation; red arrow, infiltration of numerous lymphocytes. Original magnification, ×400. Bars represent 50 µm. g C3 and IgG deposition in the glomerulus was detected by immunofluorescence and is presented in a merged manner; frozen sections of the kidney were stained with anti-mouse-C3-APC, anti-mouse-IgG-FITC, and DAPI. Original magnification, ×400. Bars represent 50 µm. The data are representative of at least two independent experiments with similar results. b, d The data were analyzed by Student’s t test, and significance is indicated by *P < 0.05